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KMID : 0545119990090030271
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 3 p.271 ~ p.275
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Purification and Characterization of Manganese Superoxide Dismutase from Staphylococcus sciuri
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Song, Chi Hyun
Park, Eun Kyung/Suh, Hyung Joo/Lee, Yong Se/Choi, Jang Won/Ra, Kyung Soo
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Abstract
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The intracellular superoxide dismutase (SOD) from Staphylococcus sciuri was isolated to homogeneity by continuous steps, including ammonium sulfate fractionation, DEAE-ion-exchange chromatography, gel filtration, and phenyl hydrophobic gel chromatography. Pure SOD had a specific activity of 4,625 U/㎎ and was purified 158-fold with a yield of 31% from a cell free extract. The molecular weight of the purified SOD was determined to be approximately 35.5 kDa by gel filtration and the enzyme was also shown to be composed of dimeric subunits on denaturing SDS-PAGE. The enzyme activity remained stable at pH 5 to 11 and also to heat treatment of up to 50℃ at pH 7.8, with 80% relative activity. The enzyme was insensitive to cyanide, hydrogen peroxide, and azide, indicating that it is a manganese-containing SOD. The EPR spectrum showed the enzyme containing manganese as a cofactor.
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